Clontech pgbkt7
WebApr 3, 2007 · Clontech Laboratories, Inc. www.clontech.com Protocol No. PT3247-1 Version No. PR742219 I. Introduction continued Optimized vectors facilitate downstream confirmation The Matchmaker System 3 DNA-BD and AD fusion vectors, pGBKT7 and pGADT7, were designed for high-level protein expression and to facilitate confirmationof … WebShow Static Map. Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. Efficient cleavage requires at least two copies of the NaeI recognition sequence. Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends ...
Clontech pgbkt7
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WebApr 11, 2024 · The yeast two-hybrid experiments were performed according to the Yeast Handbook (Clontech, USA). Briefly, full length cDNA of OsUGE1 was amplified and inserted into pGADT7 vector and pGBKT7 vector, respectively. Then fused vectors were co-transformed into yeast strain AH109 and incubated for 3 days at 30 ℃ on medium … WebClontech, Clontech Logo and all other trademarks are the property of Clontech Laboratories, Inc., unless noted otherwise. Clontech is a Takara Bio Company. 2008 Clontech Laboratories, Inc. Use: pGBKT7 is the DNA-BD Vector included with Clontech's Matchmaker Systems.
WebSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using … http://faculty.salisbury.edu/~flerickson/Seq%20and%20vectors/pgbkt7%20map.pdf
WebOct 19, 2024 · pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. WebApr 21, 2024 · Saccharomyces cerevisiae strain AH109, human fetal brain cDNA library that was constructed on pACT2 fused with the GAL4 activation domain, and control vectors pGADT7, pGADT7-T, pGBKT7-p53, and pGBKT7-Lam were from Clontech. pGBKT7-UL44 was first introduced into strain AH109 and verified that do not activate the report …
WebAbout our brands: Clontech. Our Clontech brand represents over 30 years of service to the life sciences research community through the development, commercialization, and support of kits and reagents for diverse experimental applications, with emphases in cell …
Web7 rows · pGBKT7 yeast two-hybrid bait vector. pGBKT7 is a yeast two … hsbc order annual tax statementWebJun 23, 2016 · Plasmids. Prior to performing the yeast two-hybrid assay, we constructed the bait plasmid. The full-length coding sequence of TSC22D2 was amplified by PCR and inserted into pGBKT7 (Clontech, Mountain … hsbc order checkbookWebDescription from Clontech: "pM is used to generate a fusion of the GAL4 DNA-BD (amino acids 1–147) and a protein of interest in the Matchmaker™ Mammalian Assay Kit 2 (Cat. No. 630305). The hybrid protein is targeted to the cell’s nucleus by the GAL4 nuclear … hsbc order a new cardWebpGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast … hobby lobby boise addressWeb1 day ago · To construct AD/BD recombinant vectors, the ORFs of four Aux/IAA genes (DoIAA1, 6, 10 and 13) were cloned into the pGBKT7 vector (Clontech, Palo Alto, CA, USA), and the ORFs of three ARF genes (DoARF2, 17 and 23) were cloned into the pGADT7 vector (Clontech). Two vectors (containing DoIAAs-BD and DoARFs-AD) were … hsbc ore adeyemiWebThe VP37 bait vector (pGBKT7-37) was constructed using the appropriate restriction enzyme (TaKaRa Bio Inc., Otsu, Japan), in order to clone the VP37 genes into pGBKT7 (Clontech Laboratories). hobby lobby boat toysWebDec 21, 2015 · The VP37 bait vector (pGBKT7-37) was constructed using the appropriate restriction enzyme (TaKaRa Bio Inc., Otsu, Japan), in order to clone the VP37 genes into pGBKT7 (Clontech Laboratories). The pGBKT7-37 was sequenced prior to transformation to ensure that the bait protein (VP37) encodes in the correct reading frame of the GAL4 … hobby lobby boise frames